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Bromelain treatment of human T cells
removes CD44, CD45RA, E2/MIC2, CD6, CD7, CD8, and Leu 8/LAM1
surface molecules and markedly enhances CD2-mediated T
cell activation
1Hale L.P., 2HaynesB.F.
1Department of Medicine, Division of Rheumatology
and Immunology
2Department of Microbiology and Immunology,
Duke University Medical Center, Durham, NC 27710
The Journal of Immunology 1992: Vol. 149, No. 12, pp. 3809-3816
356 KA (3-06-1) |
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Abbreviations used in this paper: LFA, lymphocyte
function-associated Ag; PB, peripheral blood.
Treatment of T cells with the cysteine protease bromelain
has been widely used to enhance the binding of human T cells
to human E (autologous E rosettes) and has been shown to
remove surface T cell CD44 molecules. Ligand binding to CD44
has been shown to markedly augment T cell activation. To
study the activation potential of bromelain-treated CD44
T cells, we have compared the proliferation of sham- and
bromelain-treated normal human PBMC to mitogenic CD2 mAb.
We found that bromelain not only removed T cell CD44, but
also removed the CD45RA isoform of CD45 as well as E2/MIC2,
CD6, CD7, CD8, and Leu 8/LAM1 molecules. T cell proliferation
in response to CD2 mAb was increased 325% in bromelain-treated
PBMC compared to sham-treated PBMC (p < 0.005). Reciprocal
treatment experiments using purified T cells and monocytes
demonstrated that the enhancement of T cell CD2 activation
by bromelain occurred only when T cells were treated with
bromelain and was accompanied by increased adhesion of T
cells to monocytes. These data demonstrate that expression
of portions of the extracellular domains of the CD44, CD45RA,
E2/MIC2, CD6, CD7, CD8, and Leu 8/LAM1 surface molecules
are not required for CD2 activation of human T cells. Rather,
the removal of these surface molecules by bromelain is associated
with enhanced T cell-monocyte aggregation and enhanced CD2-mediated
T cell activation. Taken together with data that CD44, E2/MIC2,
CD6, and CD7 mAb inhibit CD2/lymphocyte function-associated
Ag-3-mediated cellular interactions and also augment CD2-mediated
triggering of T cells, these data suggest that members of
the bromelain-sensitive group of surface molecules may comprise
a set of CD2-associated adhesion ligands that acts in concert
to modulate human T cell activation. |
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