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WAM Essentials, Inc.
Systemic Enzyme Therapy
... Allowing You to Live Your Passion!™ |
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Intestinal absorption of serrapeptase
(TSP) in rats.
Moriya N, Nakata M, Nakamura M, Takaoka M, Iwasa S, Kato
K, Kakinuma A.
Biotechnol Appl Biochem. 1994 Aug;20 ( Pt 1):101-8.
Biotechnology Research Laboratories, Takeda Chemical Industries
Ltd., Osaka, Japan. |
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| A sensitive sandwich enzyme immunoassay (e.i.a.)
for serrapeptase (TSP), an orally available anti-inflammatory
proteinase, was established using affinity-purified anti-TSP
rabbit IgG and its Fab' fragment conjugated with horseradish
peroxidase as the first and the second antibodies respectively.
TSP in the plasma was determined by the e.i.a. after its oral
administration (100 mg/kg) to rats. The peak concentration
was observed between 30 min and 2 h after administration. TSP
in the plasma samples was trapped on a microtitre plate coated
with the affinity-purified anti-TSP rabbit IgG, and the hydrolysis
of a synthetic fluorogenic substrate, butoxycarbonyl-Glu(benzyloxy)-Ala-Arg-4-methylcoumaryl-7-amide,
by the trapped TSP was fluorometrically measured (proteinase
assay). The values obtained by the e.i.a. and those obtained
by the proteinase assay correlated well for various plasma
samples. These results indicate that orally administered TSP
was absorbed from the intestinal tract and transferred into
the circulation in an enzymically active form. |
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