|
WAM Essentials, Inc.
Systemic Enzyme Therapy
... Allowing You to Live Your Passion!™ |
|
|
|
|
|
|
|
Intestinal absorption of serrapeptase
(TSP) in rats.
Moriya N, Nakata M, Nakamura M, Takaoka M, Iwasa S, Kato
K, Kakinuma A.
Biotechnol Appl Biochem. 1994 Aug;20 ( Pt 1):101-8.
Biotechnology Research Laboratories, Takeda Chemical Industries
Ltd., Osaka, Japan. |
|
A sensitive sandwich enzyme immunoassay (e.i.a.)
for serrapeptase (TSP), an orally available anti-inflammatory
proteinase, was established using affinity-purified anti-TSP
rabbit IgG and its Fab' fragment conjugated with horseradish
peroxidase as the first and the second antibodies respectively.
TSP in the plasma was determined by the e.i.a. after its oral
administration (100 mg/kg) to rats. The peak concentration
was observed between 30 min and 2 h after administration. TSP
in the plasma samples was trapped on a microtitre plate coated
with the affinity-purified anti-TSP rabbit IgG, and the hydrolysis
of a synthetic fluorogenic substrate, butoxycarbonyl-Glu(benzyloxy)-Ala-Arg-4-methylcoumaryl-7-amide,
by the trapped TSP was fluorometrically measured (proteinase
assay). The values obtained by the e.i.a. and those obtained
by the proteinase assay correlated well for various plasma
samples. These results indicate that orally administered TSP
was absorbed from the intestinal tract and transferred into
the circulation in an enzymically active form. |
|
|
Medical Disclaimer: We believe
that the role of nutrition is important to good health, however,
this web site is for informational purposes only and is not
intended to be a substitute for professional medical advice.
Our goal is to provide you, the customer, with the information
necessary for you to make important nutritional and self-help
decisions. We strongly suggest that you share any products
you decide to use with your health care professional for optimal
individual health benefits. Women who are planning on becoming
pregnant or who are pregnant should not take any supplements
without consulting their physician first. Information and statements
on this website regarding dietary supplements have not been
evaluated by the Food and Drug Administration, and are not
intended to diagnose, treat, cure, mitigate or prevent any
disease or health condition.
|
|
|
© Copyright 2004 WAM Essentials,
Inc. All Rights Reserved.
None of the material contained herein may be reproduced without
the prior written consent of WAM Essentials, Inc. |
|
|